Sanger - Low-Throughput Sequencing (One Sample) Sanger - High-Throughput Sequencing (96-well plate) Sanger - High-Throughput Ready to Load (96-well Plate) - clean up Sanger - High-Throughput Ready to Load (96-well Plate) - no clean up Due to the limitations of our capillary electrophoresis platform, we cannot successfully perform temperature-dependent analyses such as DGGE or SSCP. Please see the Sample Submission Guidelines on the CoreLims website for information about choosing size standards and primer dye labels, making a dilution plate, submitting orders and accessing analysis software. We provide one free 96-well test plate of serial dilutions to determine optimal DNA concentrations for each new customer. Please contact us if you would like to use a custom size standard. Size standards are provided by our facility and do not need to be purchased separately. Several size standards are available to analyze fragments with 3-dye or 4-dye multiplexing. Our typical turn-around time is one business day from the date of submission, and all orders are completed on a first-come, first-served basis, regardless of affiliation. For sample analysis, we provide our customers with free access to a shared copy of the GeneMapper software via remote connection to a computer at the Core Facility. Our capillary instruments can analyze fluorescently-labeled fragments up to 1200bp in length, and they provide high run-to-run consistency with low background noise. $10.00 per designed primer plus the cost of the primer and the sequencing reaction.įor Fragment Analysis, we accept unpurified (unless otherwise directed) PCR products generated by microsatellite, AFLP, RFLP, TRFLP, ARISA or other protocols.We send you the clean, full length sequence of your region of interest.We edit the sequence, design and order primers, do the sequencing reactions, then align all the sequences in Sequencher.If your DNA region of interest is longer than 800 bp, you may want to do a primer walk to sequence the entire region.Ambiguous or miscalled bases are corrected manually.Sequences are edited up to 800 bp when possible, or the end of the PCR product if it is shorter than 800 bp.You may not submit samples in individual tubes. This service is for 96-well plates only.Service includes only electrophersis on the AB 3730xl.Service includes column purification and electrophoresis on the AB 3730xl.User submits unpurified BigDye reaction.Service includes sequencing reaction, column purification and electrophoresis on the AB 3730xl.User submits custom primers or uses those provided by the Core facility.User submits purified PCR products, plasmids, cosmids, fosmids or BACs.We offer a Troubleshooting Manual on our CoreLims website to assist customers. Troubleshooting is free and we encourage customers to contact us if they are having difficulties with their sequencing project. Please see the Sample Submission Guidelines on the CoreLims website for information about sample concentration, primers, and submitting samples. All orders are completed on a first-come, first-served basis, regardless of affiliation. We offer three types of sequencing options (see below) and our typical turn-around time is one business day from the date of submission. Samples must be submitted in 96-well plate format to receive the high-throughput rate reduction. ![]() High-throughput orders are defined as 1 or more plates of sequencing reactions submitted at any one time. Low-throughput orders are defined as 1-95 sequencing reactions submitted at any one time. Our CoreLims website ( ) is used to submit and track orders, retrieve data, gain access to analysis software and obtain troubleshooting assistance.įor low- and high-throughput Sanger DNA sequencing, we accept purified PCR products, plasmids and large DNA constructs such as cosmids, fosmids and BACS. High-throughput: 1 or more 96-well plates submitted at any one time.Low-throughput: 1-95 samples submitted in tubes.These automated sequencers run with 50 cm capillary arrays and are extremely fast and accurate. We offer two levels of service options, based on the number of samples in your project and the way you submit them. All sequencing and fragment analysis samples are analyzed on two Applied Biosystems 3730xl DNA Analyzers to provide customers with the most sophisticated capillary sequencing technology to date. The Core Facility provides Sanger DNA sequencing and fragment analysis services to researchers at domestic and international academic institutions, government agencies and private companies. Please visit our CoreLims website ( ) to submit your fragment analysis orders and Sanger sequencing orders.
0 Comments
Leave a Reply. |